Adipocyte Fatty Acid Transfer Supports Megakaryocyte Maturation

Cell Rep. 2020 Jul 7;32(1):107875. doi: 10.1016/j.celrep.2020.107875. | PubMed

Colin Valet¹, Aurelie Batut¹, Alicia Vauclard¹, Alizee Dortignac¹, Marie Bellio¹, Bernard Payrastre², Philippe Valet¹, Sonia Severin³

1. INSERM U1048 and Paul Sabatier University, Institute of Cardiovascular and Metabolic Diseases, Toulouse, France.
2. INSERM U1048 and Paul Sabatier University, Institute of Cardiovascular and Metabolic Diseases, Toulouse, France; Hematology Laboratory, Toulouse University Hospital, Toulouse, France.
3. INSERM U1048 and Paul Sabatier University, Institute of Cardiovascular and Metabolic Diseases, Toulouse, France. Electronic address: sonia.severin@inserm.fr.

Abstract

Megakaryocytes (MKs) come from a complex process of hematopoietic progenitor maturation within the bone marrow that gives rise to de novo circulating platelets. Bone marrow microenvironment contains a large number of adipocytes with a still ill-defined role. This study aims to analyze the influence of adipocytes and increased medullar adiposity in megakaryopoiesis. An in vivo increased medullar adiposity in mice caused by high-fat-diet-induced obesity is associated to an enhanced MK maturation and proplatelet formation. In vitro co-culture of adipocytes with bone marrow hematopoietic progenitors shows that delipidation of adipocytes directly supports MK maturation by enhancing polyploidization, amplifying the demarcation membrane system, and accelerating proplatelet formation. This direct crosstalk between adipocytes and MKs occurs through adipocyte fatty acid transfer to MKs involving CD36 to reinforce megakaryocytic maturation. Thus, these findings unveil an influence of adiposity on MK homeostasis based on a dialogue between adipocytes and MKs.

Presented By Colin Valet